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Comparison of Conventional ELISA with Electrochemiluminescence Technology for Detection of Amyloid-β in Plasma

Year: 2010
Type: article
Abstract: Plasma amyloid-β (Aβ) level could be useful as a non-invasive biomarker in Alzheimer's disease research. We compared a multiplex electrochemiluminescence detection method with a well established ELISA method for plasma Aβ quantification. Compared to the ELISA method, the electrochemiluminescence detection method demonstrates a statistically significant, but modest correlation. The reasons for this may include the differences in the affinities of antibodies, and purity and source of Aβ peptides used as standards. However, the advantages of electrochemiluminescence detection technology include short processing time and small sample volume. This comparison demonstrates the need for a further study in optimizing this system.
DOI: 10.3233/jad-2010-100456
Authors: Esther S. Oh, Michelle M. Mielke, Paul B. Rosenberg, Alka Jain, Neal S. Fedarko, Constantine G. Lyketsos, Pankaj D. Mehta
Language: English
Subfield: Physiology
Field: Medicine
Open Access status: green
Funders: Johns Hopkins University, Rosalinde and Arthur Gilbert Foundation, National Institutes of Health, Institute for Clinical and Translational Research, University of Wisconsin, Madison, National Institute on Aging, National Institute of Neurological Disorders and Stroke
Awards: G1065891614, PET imaging of activated microglia in Alzheimer's Disease, G6063930145, G703687936, Johns Hopkins Alzheimer's Disease Research Center, G924370263
OpenAlex ID: W1590791835
FWCI: 0.95
Cites: 19
Cited by: 48
Related to: 10