Work

Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates

Year: 1968

Type: article

Abstract: Micromethods have been developed for the measurement of as little as 3 µg of chondroitin sulfate A, B, or C in mixtures with other mucopolysaccharides by the use of chondroitinase-ABC, chondroitinase-AC, chondro-4-sulfatase, and chondro-6-sulfatase. With these methods, 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone. The methods have also been utilized to determine chondroitin sulfates A, B, and C in as little as 4 ml of normal urine.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)93575-1

Authors: H Saito, T Yamagata, S Suzuki

Institution: Nagoya University

Language: English

Topic: Proteoglycans and glycosaminoglycans research

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1590922852

FWCI: 8.72

Cites: 16

Cited by: 1,369

Related to: 10