Year: 1994
Type: article
Abstract: Proliferating cell nuclear antigen (PCNA), also known as auxiliary protein of DNA polymerase 6, is involved in DNA replication and repair.Certain patients with systemic lupus erythematosus (lupus) produce autoantibodies to PCNA and the autoantibody-defined epitopes of PCNA have been inferred to be conformation-dependent.Based on antigenic properties of continuous primary structure peptides, compound peptides composed of covalently linked discontinuous sequences were synthesized and used as immunogens in rabbits.One compound peptide induced an antibody response to a nuclear antigen which demonstrated a cell cycle-related pattern of nuclear immunofluorescence with maximum expression in the DNA synthesis phase of the cell cycle associated with other features which simulated the conformation-dependent properties of lupus defined autoepitopes.Other rabbit antibodies raised against different compound peptides did not show such properties and recognized epitopes which were continuous primary sequence regions.These results show that from analysis of antigenic sites on a protein, it might be pos- sible to construct synthetic compound peptides which can mimic conformation-dependent epitopes recognized by spontaneously occurring autoantibodies.Proliferating cell nuclear antigen (PCNA)l was first identified with an autoantibody from a patient with systemic lupus erythematosus (lupus) (1) and is an acidic nuclear protein of 36 kDa which is highly expressed in proliferating cells (1, 2).I t was given the name PCNA because the nuclear protein was more highly expressed in lymphocytes after mitogenic stimulation and in cells with higher proliferative activity such as basal layer cells of the epidermis and intestinal crypt cells (1). PCNAexpression increases during late G, and early S phases of
Source: Journal of Biological Chemistry
Authors: Y. Muro, W.M. Tsai, R. Houghten, E.M. Tan
Language: English
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W1593462429