Work

Possible role for the essential GTP-binding protein Obg in regulating the initiation of sporulation in Bacillus subtilis

Year: 1995

Type: article

Abstract: We fused obg, encoding an essential GTP-binding protein in Bacillus subtilis, to the LacI-repressible, IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter Pspac. Depletion of Obg, following removal of IPTG, caused a defect in sporulation and in expression of sporulation genes that are activated by Spo0A approximately P. These defects were significantly relieved by a mutation in spo0A (rvtA11) that bypasses the normal phosphorylation pathway, indicating that Obg might normally be required, either directly or indirectly, to stimulate activity of the phosphorelay that activates Spo0A.

Source: Journal of Bacteriology

DOI: 10.1128/jb.177.11.3308-3311.1995

Authors: S J Vidwans, K Ireton, A D Grossman

Institution: Massachusetts Institute of Technology

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: G580716327, G9000345091