Work

The SGN VIGS Tool: User-Friendly Software to Design Virus-Induced Gene Silencing (VIGS) Constructs for Functional Genomics

Year: 2015

Type: article

Abstract: Virus-induced gene silencing (VIGS) is a fast and powerful method to study gene function in plants (Burch-Smith et al., 2004). It is based on plant defense mechanisms against viral gene replication and allows high-throughput silencing of genes of interest (Senthil-Kumar and Mysore, 2014). The molecular mechanisms involved in post-transcriptional gene silencing (PTGS) have been studied intensively, and its steps are well known. The silencing process begins with the recognition through Dicer-like ribonucleases (DCL) of double-stranded RNA (dsRNA) that is generated during viral replication.

Source: Molecular Plant

DOI: 10.1016/j.molp.2014.11.024

Authors: Noe Fernandez-Pozo, Hernan G. Rosli, Gregory B. Martin, Lukas A. Mueller

Institutions: Cornell University, Boyce Thompson Institute, Consejo Nacional de Investigaciones Científicas y Técnicas, National University of General San Martín, Instituto Tecnológico de Chascomús

Language: English

Topic: Plant Virus Research Studies

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $5,000

Funder: National Science Foundation