Year: 2005
Type: dissertation
Study design: Observational Study
Abstract: Background:The basic fibroblast growth factor ( bFGF) binding complex is an important mediator in wound healing and tissue repair.Aims: Assessment of the mucosal expression of bFGF, FGF receptor-1 ( FGFR-1) and syndecan-1 in patients with inflammatory bowel disease ( IBD) .Patients and Methods: Paraffin sections and frozen tissues from patients with Crohn' s disease ( CD, n=17) or ulcerative colitis ( UC, n=14) and colorectal carcinoma controls ( n=16) were evaluted for the bFGF complex using immunohistochemistry, in situ hybridization and RT-PCR.Results: Reduced immunoreactive staining for bFGF was observed in epithelial cells of IBD tissue compared to controls.Inflammatory areas in the lamina propria, however, showed an intense granular immunoreactivity for bFGF.The epithelial expression of FGFR-1 also showed a decrease with inflammation but it was strongly expressed in muscle cells of larger vessels, although less in inflamed IBD tissue.I n situ hybridization confirmed these observations, i.e., a decreased mRNA expression of bFGF and FGFR-1 in epithelial cells with a concomitant higher level in lamina propria cells in IBD.RT-PCR indicated lower bFGF and, in contrast, higher FGFR-1 mRNA levels in IBD tissues.There was a low immunoexpression of syndecan-1 in mature epithelial cells of all tissues and some higher expression in fibroblasts and macrophages of IBD tissue.Syndecan-1 mRNA was particularly present in plasma cells of IBD tissues, accompanied by a much higher mRNA level in these tissues.Conclusions: The bFGF, FGFR-1 and syndecan-1 complex is reduced in epithelial cells but, in contrast, is activated in lamina propria cells in IBD.
Author: Qiang Gao
Language: English
Topic: Inflammatory Bowel Disease
Subfield: Gastroenterology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W1594921133