Work

Three new antisera with high sensitivity to ecdysone, 3-dehydroecdysone and other A-ring derivatives: Production and characterization

Year: 2013

Type: article

Abstract: Three new antisera have been produced in rabbits immunized with E-2/3-hemisuccinate conjugates. In a competitive enzyme immunoassay (EIA) with 20-ecdysone-peroxidase as a tracer, the antisera exhibit high specificity towards the side chain of the ecdysone (E) molecule. Modifications of the A-ring do result in no or small changes of sensitivity. One antiserum (RUD-2) allows the direct quantification of total ecdysteroids in samples containing E and 3-dehydroecdysone in varying ratios, without further sophisticated sample treatment. At least one of the other antisera allows the direct quantification of mixtures of E and 25-deoxyecdysone (RUD-1). Thus, these antisera may be a useful tool in studying molting control in arthropods.

Source: KTH Publication Database DiVA (KTH Royal Institute of Technology)

Authors: Delbecque Bocking Sedlmeier Dircksen Lafont, U J P D D H R Von Gliscynski

Language: English

Topic: Neurobiology and Insect Physiology Research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W1596417380

FWCI: 0.12

Cites:

Cited by: 13

Related to: 10