Work

The Control of Ribonucleic Acid Synthesis in Escherichia coli

Year: 1970

Type: article

Abstract: The preceding paper in this series presented evidence implicating an unusual phosphorylated compound (MS I) as a possible inhibitor of RNA accumulation during amino acid starvation of stringent strains of Escherichia coli. Here a method is described for isolating MS I from batch cultures of a stringent valyl transfer RNA synthetase mutant. Chemical analyses indicate that MS I is a tetraphosphate of guanosine. However, in contrast to previously described isomers of guanosine tetraphosphate (ppppG and pppGp), MS I is labile in alkali. Charge considerations, enzyme degradation studies, and labilities in acid and alkali lead to a structural assignment for MS I of guanosine 5'-diphosphate or 3'- or 2'-diphosphate (ppGpp).

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)63153-9

Authors: Michael Cashel, Barbara Kalbacher

Institutions: National Institutes of Health, United States Public Health Service, National Institute of Neurological Disorders and Stroke

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1597428765

FWCI: 28.88

Cites: 55

Cited by: 374

Related to: 10