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Stranded Whole Transcriptome RNA‐Seq for All RNA Types

Year: 2015
Type: article
Abstract: Stranded whole transcriptome RNA-Seq described in this unit captures quantitative expression data for all types of RNA including, but not limited to, miRNA (microRNA), piRNA (Piwi-interacting RNA), snoRNA (small nucleolar RNA), lincRNA (large non-coding intergenic RNA), SRP RNA (signal recognition particle RNA), tRNA (transfer RNA), mtRNA (mitochondrial RNA), and mRNA (messenger RNA). The size and nature of these types of RNA are irrelevant to the approach described here. Barcoded libraries for multiplexing on the Illumina platform are generated with this approach but it can be applied to other platforms with a few modifications.
DOI: 10.1002/0471142905.hg1114s84
Authors: David F.B. Miller, Pearlly X. Yan, Fang Fang, Aaron Buechlein, James B. Ford, Haixu Tang, Tim H. Huang, Matthew E. Burow, Yunlong Liu, Douglas B. Rusch, Kenneth P. Nephew
Institutions: Indiana University Bloomington, Indiana University School of Medicine, Indiana University, The Ohio State University Wexner Medical Center, The University of Texas at San Antonio Health Science Center, Tulane University, Indiana University Indianapolis
Language: English
Open Access status: green
OpenAlex ID: W1598478521
FWCI: 0.723
Cites: 11
Cited by: 8
Related to: 10