Work

Quantitative measurement of the total, peroxyl radical‐trapping antioxidant capability of human blood plasma by controlled peroxidation: The important contribution made by plasma proteins

Year: 1985

Type: article

Abstract: Plasma obtained from fasted humans has been analysed for total radical-trapping antioxidant content by subjecting it to controlled peroxidation using the thermal decomposition of water-soluble azobis (2-amidinopropane hydrochloride) at 37 degrees C to produce peroxyl radicals at a known, steady rate. It is found that the total radical-trapping antioxidant content is rather similar for the 7 subjects that have been tested and, furthermore, it is 10-20-times larger than the effect attributable to vitamin E alone. Although it is shown that urate and ascorbate augment the contribution from vitamin E, their contributions (21-34 and 0-2%, respectively) still leave 57-73% of the total antioxidant content unaccounted for. Evidence is presented to show that this previously unrecognized large reserve of antioxidant capacity is attributable to the plasma proteins.

Source: FEBS Letters

DOI: 10.1016/0014-5793(85)81208-4

Authors: D.D.M. Wayner, G.W. Burton, K.U. Ingold, S. Locke

Institutions: National Research Council Canada, Mount Allison University

Language: English

Topic: Antioxidant Activity and Oxidative Stress

Subfield: Biochemistry (medical)

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Foundation for Cancer Research