Work

Cholesterol is Required for the Formation of Regulated and Constitutive Secretory Vesicles from the trans‐Golgi Network

Year: 2000

Type: article

Abstract: We studied the role of cholesterol in regulated protein secretion in neuroendocrine cells by manipulating the cholesterol content of AtT-20 cells. Depletion of cellular cholesterol levels caused a reversible block of immature secretory granule biogenesis at the level of the trans-Golgi-network, whereas increased cholesterol levels promoted immature secretory granule formation. Cholesterol depletion also blocked the formation of constitutive secretory vesicles, but did not inhibit the transport between the endoplasmic reticulum and the Golgi complex. Our results indicate that the assembly of cholesterol-based lipid microdomains is required for the biogenesis of both regulated and constitutive secretory vesicles from the trans-Golgi-network in neuroendocrine cells.

Source: Traffic

DOI: 10.1034/j.1600-0854.2000.011205.x

Authors: Yanzhuang Wang, Christoph Thiele1, Wieland B. Huttner

Institutions: Heidelberg University, Max Planck Institute of Molecular Cell Biology and Genetics

Language: English

Topic: Cellular transport and secretion

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Deutsche Forschungsgemeinschaft, German-Israeli Foundation for Scientific Research and Development