Work

Endothelial Cell Stimulation by Candida albicans

Year: 2008

Type: book-chapter

Abstract: The opportunistic fungal pathogen Candida albicans enters the bloodstream and causes hematogenously disseminated infection in hospitalized patients. During the initiation of a hematogenously disseminated infection, endothelial cells are one of the first host cells to come in contact with C. albicans. Endothelial cells can significantly influence the local host response to C. albicans by expressing leukocyte adhesion molecules and proinflammatory cytokines. Thus, it is of interest to investigate the response of endothelial cells to C. albicans in vitro. We describe the use of real-time PCR and enzyme immunoassays to measure the effects of C. albicans on the endothelial cell production of E-selectin and tumor necrosis factor alpha in vitro.

Source: Methods in molecular biology

DOI: 10.1007/978-1-59745-204-5_22

Authors: Quynh T. Phan, Scott G. Filler

Institutions: University of California, Los Angeles, The Lundquist Institute, Harbor–UCLA Medical Center

Language: English

Topic: Antifungal resistance and susceptibility

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institute of Allergy and Infectious Diseases, National Center for Research Resources