Work

Pillars article: Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry. Science 1992. 255: 1261-1263.

Year: 2007

Type: article

Abstract: Antigens recognized by T cells are expressed as peptides bound to major histocompatibility complex (MHC) molecules. Microcapillary high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry was used to fractionate and sequence subpicomolar amounts of peptides isolated from the MHC molecule HLA-A2.1. Of 200 different species quantitated, eight were sequenced and four were found in cellular proteins. All were nine residues long and shared a distinct structural motif. The sensitivity and speed of this approach should enhance the analysis of peptides from small quantities of virally infected and transformed cells as well as those associated with autoimmune disease states.

Source: PubMed

Authors: Donald F Hunt, Robert A Henderson, Jeffrey Shabanowitz, Kazuyasu Sakaguchi, Hanspeter Michel, Noelle Sevilir, Andrea L Cox, Ettore Appella, Victor H Engelhard

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases

Awards: Antigenic determinants on HLA-B7 and-A2, Processed Antigen Characterization by Mass Spectrometry, ANTIGENIC DETERMINENTS ON HLA-B7 AND HLA-A2, Protein Sequencing by Tandem Mass Spectrometry

OpenAlex ID: W1603286413

FWCI: 0.145

Cites: 28

Cited by: 9

Related to: 10