Work

Dynamic Imaging Using Fluorescence Resonance Energy Transfer

Year: 2002

Type: article

Abstract: Recently, fluorescence resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) have been the buzzwords for monitoring protein-protein interactions in living cells.These methodologies provide higher temporal (< 1 ns) and spatial resolution (1-10 nm) than near field microscopy (50 nm).FRET microscopy is an excellent method for imaging the structure and localization of proteins under physiological conditions, since other methods, including X-ray diffraction and electron microscopy, require the specimens to be fixed.

Source: BioTechniques

DOI: 10.2144/02326im01

Authors: Masilamani Elangovan, Richard N. Day, Ammasi Periasamy

Institution: University of Virginia

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,415

Funder: W. M. Keck Foundation