Work

Glucocorticoid-binding Macromolecules in Rat and Mouse Thymocytes

Year: 1973

Type: article

Abstract: The physicochemical properties of glucocorticoid receptors were studied in rat and mouse thymocytes.The steroidreceptor complex formed when thymocytes were incubated with [1,2 ,4-3H]triamcinolone acetonide ([3H]TA) was assayed in a 27,000 x g supernatant fraction using sucrose density gradient analysis.After incubation of rat thymocytes at 0" for 30 min most of the bound radioactivity was associated with the 27,000 x g supernatant obtained by extracting the cells with a buffer containing a low concentration of salt (<O.OOl M); two [3H]TA-receptor complexes which sedimented at -3.5 S and -7 S were observed in this fraction.At O', the buffer containing high salt (0.15 M KCl) extracted [3H]TA-receptor complex sedimenting at -4 S. When incubated at 37" for 30 min, the bulk of bound radioactivity was found in the 27,000 x g pellet from which it could be extracted only with high salt buffer to give a [3H]TA-receptor complex which sedimented at -4 S. Mouse thymocytes differed from rat thymocytes in that following incubation with [3HJTA at 0" for 30 min and extraction with the low salt buffer, they yielded only one complex sedimenting at -7 S. Pretreatment in vivo with TA increased the in vifro binding of the [3H]TA to the 3.5 S receptor at the expense of binding to the 7 S receptor.It is concluded that the qualitative distribution of the glucocorticoid receptors is species specific and is dependent on the incubation temperature and the ionic strength of the buffer used for extraction and sucrose density analysis.Certain normal and malignant lymphoid tissues undergo regression and dissolution follov,ing glucocorticoid treatment in vivo (l-3).In previous studies concerned with the mechanism of this hormonal response, we have used the glucocorticoidsensitive and -resistant lines of mouse lymphosarcoma I'1798

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(19)44399-8

Authors: Nurit Kaiser, Richard J. Milholland, Fred Rosen

Institutions: University at Buffalo, State University of New York, Roswell Park Comprehensive Cancer Center, New York State Department of Health

Language: English

Topic: Estrogen and related hormone effects

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,430