Work

Purification and interaction properties of the human RNA polymerase B(II) general transcription factor BTF2.

Year: 1991

Type: article

Abstract: A general transcription factor (BTF2) has been purified from HeLa whole cell extract and shown to be absolutely required for the formation of a functional initiation complex. We also demonstrate that this factor binds in solution to RNA polymerase B(II) and to the other general transcription factors BTF1(TFIID), BTF3, and BTF4. These results strongly suggest the existence of multiple interactions between the various components of the multiprotein initiation complex.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)54801-8

Authors: M. Gerard, L. Fischer, V. Moncollin, J.M. Chipoulet, P. Chambon, J.M. Egly

Institution: Inserm

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1606394252

FWCI: 4.005

Cites: 39

Cited by: 204

Related to: 10