Work

Deoxynucleotide-polymerizing Enzymes of Calf Thymus Gland

Year: 1971

Type: article

Abstract: A simplified procedure for purification of terminal deoxynucleotidyl transferase is presented. The enzyme is shown to be homogeneous by equilibrium centrifugation and gel electrophoresis. The molecular weight of the enzyme is 32,360, with a v measured as 0.65 cc g-1. The homogeneous enzyme can be dissociated into two subunits, α and β, by sodium dodecyl sulfate. Subunit molecular weights, estimated from gel electrophoresis, are α = 8,000 and β = 26,500.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)62410-x

Authors: Lucy M.S. Chang, F.J. Bollum

Institution: University of Kentucky

Language: English

Topic: Biochemical and Molecular Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1607917106

FWCI: 7.076

Cites: 49

Cited by: 305

Related to: 10