Work

In Vitro Synthesis of Ribosomal Ribonucleic Acid by a Deoxyribonucleic Acid-Protein Complex Isolated from Escherichia coli

Year: 1973

Type: article

Abstract: A DNA-RNA polymerase complex has been isolated from EDTA-treated Escherichia coli MRE 600. This fraction contains virtually all of the RNA synthetic capacity expressed in the EDTA-treated cells. The in vitro synthesis is sensitive to rifampicin, indicating that the complex is actively initiating RNA chains. Analysis of the product RNA by hybridization competition shows that between 20 to 30% is rRNA. Guanosine tetraphosphate (ppGpp) inhibits global RNA and rRNA synthesis equally, and no preferential inhibition of rRNA synthesis is detected.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(19)43536-9

Authors: Yoshikatsu Murooka, Robert A. Lazzarini

Institutions: National Institutes of Health, National Institute of Neurological Disorders and Stroke

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1608095408

FWCI: 1.945

Cites: 13

Cited by: 19

Related to: 10