Work

Isolation and Screening of Marine Microalgae Chlorella Sp. _pr1 for Anticancer Activity

Year: 2014

Type: article

Abstract: Objective: The objective of the present study includes isolation, characterization and screening of anticancerous activity against B16F10 cell line using isolated marine microalgae Chlorella sp._PR1. Methods: In this study, marine microalgae Chlorella sp._PR1 isolated and cultured using f/2 medium and anticancerous activity was assayed using MTT assay. Results: The DMSO extract of Chlorella sp._PR1 was exhibit anticancerous activity against murine melanoma B16F10 cell line. The extract exhibit reduction of cell viability up to 56% with 2µg/ml concentration. IC 50 were calculated and was found that Chlorella sp._PR1 need 5.5 μg/ml of the compounds to reduce the murine melanoma B16F10 cell viability by 50%. Fluorescence activated cell sorting (FACS) analysis revealed that Chlorella sp._PR1 extract (8 µg/ml) brought significant inhibition (p<0.01) of the G 0 -G 1 and the S phase. The extract did not seem to affect the G 2 -M phase. Conclusion: DMSO extract of Chlorella sp._PR1 (5.5 µg/ml) was found to be potent against murine melanoma B16F10 cell line.

Source: International Journal of Pharmacy and Pharmaceutical Sciences

Authors: Pratibha Gupta, Dona Sinha, Rajib Bandopadhyay

Institutions: Birla Institute of Technology, Mesra, Chittaranjan National Cancer Institute

Language: English

Topic: Seaweed-derived Bioactive Compounds

Subfield: Aquatic Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W1609777439

FWCI: 1.167

Cites: 12

Cited by: 10

Related to: 10