Year: 2013
Type: article
Abstract: The precise control of gene expression is critical in embryonic development. Quantitative assays, such as microarrays and RNA sequencing, provide gene expression levels for a large number of genes, but do not contain spatial information. In contrast, in situ methods, such as in situ hybridization and immunohistochemistry, provide spatial resolution, but poor quantification and can only reveal the expression of one, or very few genes at a time. Furthermore, the usual methods of documenting the results, by photographing whole mounts or sections, makes it very difficult to assess the three-dimensional (3D) relationships between expressing and nonexpressing cells. Optical projection tomography (OPT) can capture the full 3D expression pattern in a whole embryo at a reasonable level of resolution and at moderately high throughput. A large database containing spatio-temporal patterns of expression for the mouse (e-Mouse Atlas Project, EMAP, www.emouseatlas.org) has been created, incorporating 3D information. Like the mouse, the chick is an important model in developmental biology and translational studies. To facilitate comparisons between these important model organisms, we have created a 3D anatomical atlas, accompanied by an anatomical ontology of the chick embryo and a database of gene expression patterns during chick development. This database is publicly available (www.echickatlas.org).
Source: genesis
DOI: 10.1002/dvg.22374
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Awards: G1252325381, A three-dimensional atlas of gene expression during chick development with cross comparisons to the mouse, Investigation of the role of the primary cilia and centrosome during signalling events in development using the chicken mutant talpid3, Transcriptomes, networks and systems, Bioinformatic analysis, National Capability Grant - National Avian Research Facility, A three-dimensional atlas of gene expression during chick development with cross comparisons to the mouse, National Capability Grant - Edinburgh Genomics, Future-Proofing the Sustainability of the MRC High Throughput Sequencing Hub in Scotland, Increase understanding tissue development to improve production and health, A three-dimensional atlas of gene expression during chick development with cross comparisons to the mouse
OpenAlex ID: W1614089981