Proteinaceous Compounds From Fragaria Ananassa Fruit Attenuates Paraquat Induced Parkinson Like Locomotor and Mitochondrial Alterations in Zebrafish
Year: 2015
Type: article
Abstract: Objectives: To assess the Parkinson like locomotor and mitochondrial alterations, associated with the exposure of paraquat (PQ), in vivo preventive effect of proteinaceous compounds extracted from Fragaria ananassa fruit (FA-D) against mitochondrial dysfunction induced by paraquat in zebra fish using brain mitochondria. Methods : Parkinson like locomotor and mitochondrial alterations were resulted by intra peritoneal administration of 55 mM PQ alternatively for a period of 7 days. The water soluble proteinaceous compounds from Fragaria ananassa fruit were obtained by Ammonium sulphate fractionation. The molecular weight of FA-D fraction was determined by SDS-PAGE and we found three distinct bands at 20.0 kDa, 17.0 kDa and 14.4.0 kDa bands respectively. The in vitro antioxidant activity and the in vivo preventive effect of FA-D against PQ induced Parkinsonian symptoms was evaluated by different assay systems viz., in vitro : radical scavenging activity by DPPH reaction and in vivo : locomotion, dopamine levels, complex-I activity, mitochondrial ROS levels, cytochrome c release and mitochondrial morphology. Results : The results show that paraquat altered locomotor activity and increased dopamine levels. Mitochondria isolated from paraquat treated zebrafish showed a marked inhibition of complex-I activity, increase in mitochondrial reactive oxygen species (mt ROS) and cytochrome c release and disintegration of mitochondrial structure. Treatment of 0.25 mg/kg body weight of FA-D fraction once in alternative days, for 5 days subsequent to the administration of PQ alternatively for a period of 7days, substantially reduced mt ROS levels and markedly restored the complex-I activity, cytochrome c release and mitochondrial morphology. Conclusion: The results strongly suggest that proteinaceous compounds from Fragaria ananassa fruit recuperate paraquat induced Parkinsonian like symptoms by protecting the mitochondria.
Institution: Sathyabama Institute of Science and Technology
Language: English
Subfield: Clinical Neurology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W1627590033