Effect of dimethyl sulfoxide (DMSO), sodium hydroxide (NaOH), acetone, and ethanol on the population growth, mictic female production, and body size of the rotifer Brachionus plicatilis Muller
Year: 1997
Type: article
Abstract: Dimethyl sulfoxide (DMSO), sodium hydroxide (NaOH), acetone, and ethanol which are commonly used as solvents for steroid and thyroid hormones were tested for their effect on rotifer population growth, mictic female production, and body size.Each chemical was tested at 0.2%, 0.4%, 0.6%, 0.8% and 1% of the 5-ml Nannochloropsis oculata suspension (7 x 106 cells/ml).Initially, 5 rotifers each carrying one amictic egg were exposed in these concentrations at 25°C in dark condition for 48 hours.Thereafter, rotifers were counted and transferred to a new culture medium without the chemical on day 2, 4, 6, and 8. Body size was measured on day 8. DMSO at 0.2% and 0.4% significantly increased rotifer population growth while 0.4, 0.6, 0.8, and 1% caused an increase in mictic female production.NaOH at 0.4% and 0.8% significantly increased population growth while 0.8% caused an increase in mictic female production.Acetone at 0.8% and 1% caused a decrease in population growth but it had no effect on mictic female production.Ethanol caused a decrease in population growth in all tested concentrations but it did not affect mictic female production.Body size of DMSO-, NaOH-, acetone-, or ethanol-treated rotifers was not significantly different from that of the control.Acetone may be used as a solvent at concentrations lower than 0.8.%, whereas DMSO and NaOH may be used as high as 1% without adverse effects.Ethanol caused adverse effects as low as 0.2% and is therefore not suitable as solvent in experiments with rotifer populations.Rotifera, Brachionus plicatilis, dimethyl sulfoxide, sodium hydroxide, acetone, ethanol, hormone solvents, population growth, mixis, body size
Authors: Gallardo, Wenresti G., Tomita, Yuichi, Hagiwara, Astushi, Soyano, Kiyoshi, Snell, Terry W.
Institutions: Nagasaki University, Georgia Institute of Technology
Language: English
Subfield: Health, Toxicology and Mutagenesis
Field: Environmental Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W162761970