NECROTISING HEPATOBACTERIUM (NHPB) INFECTION IN Penaeus vannamei WITH FLORFENICOL AND OXYTETRACYCLINE: A COMPARATIVE EXPERIMENTAL STUDY Infección Experimental de Hepatobacteria Necrotizante (NHPB) en Penaeus vannamei con florfenicol y oxitetraciclina: un estudio experimental comparativo
Year: 2012
Type: article
Abstract: The present study is aimed to necrotising hepatobacterium\n(NHPB) infection, development in juvenile of Penaeus vannamei\nwith florfenicol (FF) (1.000g/kg biomass) and oxytetracycline\n(OTC) (6.070 g/kg biomass). HPLC analysis was used to confirm\nthe antibiotics in food and samples, wet mount analysis, conventional\nhistopathology, PCR and in situ hybridization were used to\nassess the prevalence, mortality and severity of NHP and to confirm\nNHPB infection. Wet-mount analysis and histopathological\nresults demonstrated that the Penaeus vannamei fed with OTC\nhad 100% NHPB prevalence disease severity index 1 (10%), 2\n(28%), 3 (35%) and 4 (27%); meanwhile, P. vannamei fed with\nFF had 100% NHPB prevalence at disease severity index 1\n(16%), 2 (36%), 3 (20%) and 4 (28%). The positive control had\ndisease severity index 1 (10%), 2 (10%), 3 (80%) and 4 (0%); no\ndisease NHP-B signs were revealed by the negative control. A\nweak positive signal was shown by the in situ hybridization from\nthe 9th day, and a positive signal from the 15nd day. The results\nderived by the High-performance liquid chromatography (HPLC)\nanalysis demonstrated that the maximum OTC level was in muscle\n(on the 6th and 7th day, respectively) and the FF level in hepatopancreas\n(HP), followed by muscle. It was conclude that FF\nand OTC used in medicated feed as an effective treatment in the\ncontrol of NHPB disease in P. vannamei when the medication is\nsupplied in disease severity index 1 and 2.
Institution: Universidad Mesoamericana
Language: Spanish
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W1631861504