Work

The Dissociation Constants of Tetrahydrofolic Acid

Year: 1966

Type: article

Abstract: Six dissociable groups exist in tetrahydrofolic acid in the range H0 -3.0 to pH 14. The assignments in tetrahydrofolic acid by titrimetric and spectrophotometric comparisons with model compounds, p-aminobenzoylglutamic acid and 2-amino-6,7-dimethyl-5,6,7,8-tetrahydro-4-pteridinone, are as follows: acidic ionization of amide, pK' 10.5; N5, pK' 4.82; γ-carboxyl, pK' 4.8; α-carboxyl, pK' 3.5; N1, pK' 1.24; and N10, pK' -1.25. These pK' values, with the exception of those of the carboxyl groups, were determined directly for tetrahydrofolic acid at 22–25° and ionic strength 1.0.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)96349-0

Authors: Roland G. Kallen, William P. Jencks

Institution: Brandeis University

Language: English

Topic: Folate and B Vitamins Research

Subfield: Rheumatology

Field: Medicine

Domain: Health Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1636203097

FWCI: 1.422

Cites: 38

Cited by: 120

Related to: 10