Ribonucleic Acid Chain Elongation by Escherichia coli Ribonucleic Acid Polymerase
Year: 1974
Type: article
Abstract: The chain elongation portion of enzymatic RNA synthesis has been studied by employing a ternary complex containing RNA polymerase, a DNA template, and product RNA. Ternary complexes were isolated by passage through a gel exclusion column and were shown to carry out RNA chain elongation under conditions that preclude RNA chain initiation or termination. The properties of the elongation reaction have been studied by employing ternary complexes formed with synthetic DNA templates. A simple ping-pong kinetic model was derived and was shown to fit the data obtained with alternating copolymer templates. The model is also able to account for the inhibition observed at high concentrations of nucleoside triphosphates. A general rate equation can be devised for RNA chain elongation with DNA templates of complex sequence if one assumes that the Michaelis constants for the substrates are independent of the DNA sequence. This assumption appears to hold for the various synthetic DNA templates that we have examined but has not been adequately tested for more complex templates.
Source: Journal of Biological Chemistry
Authors: Gary Rhodes, Michael J. Chamberlin
Institution: University of California, Berkeley
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W1646260588