Work

Construction of a Human Antibody Domain (VH) Library

Year: 2008

Type: book-chapter

Abstract: Highly diverse antibody (Fab or scFv) libraries have become vital sources to select antibodies with high affinity and novel properties. Combinatorial strategies provide efficient ways of creating antibody libraries containing a large number of individual clones. These strategies include the reassembly of naturally occurring genes encoding the heavy and light chains from either immune or nonimmune B-cell sources, or introduction of synthetic diversity to either the framework regions (FRs) or the complementarity-determining regions (CDRs) of the variable domains of antibodies. In the late 1980s, the smallest known antigen-binding fragment was identified when a murine VH repertoire was screened for binding to lysozyme. This fragment (approximately 15 kDa), called a "domain antibody", or "dAb", is approximately four times smaller than a Fab and half the size of a scFv. Here, we describe the construction of a phage-displayed VH library and an approach to introduce genetic diversity in this library, where both diverse human CDRs and synthetic CDRs are combined into a single-domain (VH) framework.

Source: Methods in molecular biology

DOI: 10.1007/978-1-59745-554-1_4

Authors: Weizao Chen, Zhongyu Zhu, Xiaodong Xiao, Dimiter S. Dimitrov

Institutions: National Institutes of Health, National Cancer Institute

Language: English

Topic: Monoclonal and Polyclonal Antibodies Research

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: Cancer Research Institute, National Institutes of Health, National Cancer Institute

Award: Human Monoclonal Antibodies and Antibody-Guided Nanoparticles