Insecticide susceptibility of A nopheles coluzzii and A nopheles gambiae mosquitoes in I badan, Southwest N igeria
Year: 2014
Type: article
Abstract: The emergence of insecticide resistance in Anopheles (Diptera: Culicidae) mosquitoes has great implications for malaria control in Nigeria. This study aimed to determine the dynamics of insecticide susceptibility levels and the frequency of knock-down resistance (kdr) mutations (L1014F) in wild Anopheles coluzzii Coetzee & Wilkerson sp. n. and Anopheles gambiae Giles from the Ojoo and Bodija areas of Ibadan, in southwest Nigeria. Insecticide susceptibility to pyrethroids, organophosphates, carbamates and organochlorines was assessed using World Health Organization (WHO) bioassays. A subset of the mosquitoes exposed to pyrethroids and DDT was used for species and molecular form identification; kdr genotyping was determined using the TaqMan real-time polymerase chain reaction assay. The mosquitoes were resistant to pyrethroids and DDT but completely susceptible to organophosphates and carbamates. Bodija samples (n = 186) consisted of An. gambiae (91.4%) and An. coluzzii (8.1%) and included one An. coluzzii/An. gambiae hybrid specimen. All mosquitoes screened in Ojoo (n = 26) were An. gambiae. The 1014F kdr mutation was detected at frequencies of 24.5 and 5.8% in Bodija and Ojoo, respectively. No correlation was observed between kdr genotypes and resistance phenotypes. The results indicate that metabolic resistance probably plays an important role in the development of resistance and highlight the need to implement insecticide resistance management strategies.
DOI: 10.1111/mve.12089
Authors: P. N. OKORIE, O. G. ADEMOWO, H. IRVING, L. A. KELLY‐HOPE, C. S. WONDJI
Language: English
Topic: Malaria Research and Control
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: Medical Education Partnership Initiative in Nigeria, Characterization of pyrethroid resistance in field populations of Anopheles funestus, malaria vector in Africa., Tackling insecticide resistance in the major African malaria vector Anopheles funestus: developing new molecular diagnostic tools, understanding the evolution of resistance and its impact on control interventions.
OpenAlex ID: W1660474053