Antidiabetic Activity of Seed of Eugenia Jambolana in Streptozotocin Induced Diabetic Male Albino Rat: an Apoptotic and Genomic Approach
Year: 2014
Type: article
Abstract: Objective : The study aimed to investigate the antidiabetic effect of ethyl acetate fraction of seed of Eugenia jambolana ( E. jambolana ) at genomic level in streptozotocin (STZ) induced diabetic male albino rat. Methods : Diabetic rats were treated with said fraction at the dose of 200 mg/Kg of body weight/day for 35 days. Potential antidiabetic mechanisms were investigated with blood glucose (short duration and long duration model), serum insulin, haemoglobin A 1 C (HbA 1 C), ISEL (In-Situ End Labelling) study of pancreatic tissue and quantitative RT-PCR study of hepatic hexokinase-I (Hex-I), Bax and Bcl-2 gene expression. Results : Results showed a significant antihyperglycemic action of the said fraction in both short and long duration treatment schedule. Serum insulin and HbA 1 C levels were also recovered in treated group in compare to the untreated diabetic group (p<0.05). ISEL study focused the regeneration of pancreatic beta cells in treated group. It was also observed that the correction in expression in Bax, Bcl-2 and Hex-I gene in hepatic tissue after the treatment of the fraction in the diabetic rat. The antidiabetic activity of the fraction was compared with glibenclamide, a standard antidiabetic drug. Conclusion : The findings provide information about the antihyperglycemic activity of this fraction through gene regulation.
Institution: Vidyasagar University
Language: English
Subfield: Endocrinology, Diabetes and Metabolism
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W1660647132