Work

Overview: Engineering Transgenic Constructs and Mice

Year: 2009

Type: article

Abstract: Cell biology research encompasses everything from single cells to whole animals. Recent discoveries concerning particular gene functions can be applied to the whole animal for understanding genotype-phenotype relationships underlying disease mechanisms. For this reason, genetically manipulated mouse models are now considered essential to correctly understand disease processes in whole animals. This unit reviews the basic mouse technologies used to generate conventional transgenic mice, which represent a gain-of-function approach. First, an overview of transgenic construct design is presented. This unit then explains basic strategies for the identification and establishment of independent transgenic mouse lines, followed by comments on historical and emerging techniques. It then describes typical problems that are encountered when researchers start to generate transgenic mice.

Source: Current Protocols in Cell Biology

DOI: 10.1002/0471143030.cb1910s42

Authors: Naoto Haruyama, Andrew Cho, Ashok B. Kulkarni

Institutions: National Institutes of Health, National Institute of Dental and Craniofacial Research

Language: English

Topic: Animal Genetics and Reproduction

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institutes of Health

Award: G8000298485