Work

The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins

Year: 2008

Type: book-chapter

Abstract: Biochemical assay of proteomic libraries derived from the Saccharomyces cerevisiae genome provides a powerful new tool for the assignment of activities to proteins. Particular advantages of this approach include the speed with which a protein can be identified and the generality for any biological activity for which an assay can be developed. We discuss the utility of this approach for the identification of RNA-modifying enzymes using a yeast proteomic library derived from a genomic set of strains expressing GST-ORF fusion proteins. This technique is also broadly applicable to other classes of RNA-protein interactions, including RNA binding and RNA degradation, and can be used with any of the proteomic libraries that are available.

Source: Methods in molecular biology

DOI: 10.1007/978-1-60327-475-3_25

Authors: Jane E. Jackman, Elizabeth J. Grayhack, Eric M. Phizicky

Institutions: University of Rochester Medicine, University of Rochester

Language: English

Topic: RNA Research and Splicing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: G3509585538, tRNA Processing