Work

Global assessment of cross-hybridization for oligonucleotide arrays.

Year: 2006

Type: article

Abstract: Cross-hybridization is the tendency for chains of nucleic acids to bind to other chains of nucleic acids that have similar but not identical sequences. This has the potential to make the interpretation of microarray experiments difficult since intensity at a spot on the array does not simply depend on the quantity of target in the sample. We propose a method for evaluating the extent of cross-hybridization in oligonucleotide arrays for data arising from a typical microarray experiment. We show that the level of cross-hybridization can be quite substantial. We argue that this makes the interpretation of the difference calls provided by MAS 5.0 difficult.

Source: PubMed

Authors: Cavan Reilly, Arvind Raghavan, Paul Bohjanen

Institution: University of Minnesota

Language: English

Topic: Gene expression and cancer classification

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Cancer Institute, National Institute of Allergy and Infectious Diseases

Awards: RNA-BP in Regulation of T Lymphocyte Activation, GREAT LAKES REGIONAL CENTER FOR AIDS RESEARCH, Regulation of T Lymphocyte mRNA Degradation

OpenAlex ID: W167753311

FWCI: 0.225

Cites: 16

Cited by: 7

Related to: 10