Biodegradable alginate microspheres as a delivery system for naked DNA.
Year: 1999
Type: article
Abstract: Sodium alginate is a naturally occurring polysaccharide that can easily be polymerized into a solid matrix to form microspheres. These biodegradable microspheres were used to encapsulate plasmid DNA containing the bacterial beta-galactosidase (LacZ) gene under the control of either the cytomegalovirus (CMV) immediate-early promoter or the Rous sarcoma virus (RSV) early promoter. Mice inoculated orally with microspheres containing plasmid DNA expressed LacZ in the intestine, spleen and liver. Inoculation of mice with microspheres containing both the plasmid DNA and bovine adenovirus type 3 (BAd3) resulted in a significant increase in LacZ expression compared to those inoculated with microspheres containing only the plasmid DNA. Our results suggest that adenoviruses are capable of augumenting transgene expression by plasmid DNA both in vitro and in vivo.
Source: PubMed
Authors: N Aggarwal, H HogenEsch, P Guo, A North, M Suckow, S K Mittal
Institutions: Purdue University West Lafayette, University of Notre Dame
Language: English
Subfield: Pharmaceutical Science
Domain: Life Sciences
Open Access status: green
Funder: National Institutes of Health
OpenAlex ID: W169223693