Work

Metabolism of hyperpolarized [1‐13C]pyruvate in the isolated perfused rat lung – an ischemia study

Year: 2012

Type: article

Abstract: We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1-(13) C pyruvate to compare the rate of lactate labeling differing only in the temporal ordering of ischemic and normoxic acquisitions. In both cases, a several-fold increase in lactate labeling was observed immediately after a 25-min ischemia event as was its reversal back to the baseline after 30-40 min of resumed perfusion (n = 5, p < 0.025 for both comparisons). These results were corroborated by (31) P spectroscopy and correspond well to measured changes in lactate pool size determined by (1) H spectroscopy of freeze-clamped specimens.

Source: NMR in Biomedicine

DOI: 10.1002/nbm.2777

Authors: B. Pullinger, H. Profka, J. H. Ardenkjaer‐Larsen, N. N. Kuzma, S. Kadlecek, R. R. Rizi

Institution: University of Pennsylvania

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Institute of Biomedical Imaging and Bioengineering