Work

An agarose-based cloning-ring anchoring method for isolation of viable cell clones

Year: 2009

Type: article

Abstract: Isolation of clonal cell populations is a crucial aspect of cell biology during engineering of specific cell strains with both genotypic and phenotypic variations. The use of cloning rings is the most established method, but requires anchoring chemicals or material that can often interfere with quantitative clonal-cell isolation and causes physical damage to the cells. Here we report a non-toxic, cell culture-compatible method that uses aga-rose for embedding the cloning rings during isolation of cell clones from monolayer cultures, with enhanced cell-viability and reproducibility during downstream applications. The method is simple and rapid, minimizing the chances for desiccation or cross-contamination during colony-lifts.

Source: BioTechniques

DOI: 10.2144/000113079

Authors: Saroj P. Mathupala, Andrew E. Sloan

Institutions: Wayne State University, The Barbara Ann Karmanos Cancer Institute, University Hospitals Cleveland Medical Center, Case Comprehensive Cancer Center

Language: English

Topic: Viral Infectious Diseases and Gene Expression in Insects

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,415

Funders: U.S. Public Health Service, National Cancer Institute