LDL from Familial Hypercholesterolemic Subjects Promotes Arterial Smooth Muscle Cell Proliferation
Year: 1994
Type: article
Abstract: To elucidate the mechanism of low-density lipoprotein (LDL) from familial hypercholesterolemic (FH) human subjects on the cultured smooth muscle cell (SMC), cell proliferation, Ca2+ movements and membrane structure were examined, along with the antiproliferative action of heparin or sulfated glycosaminoglycans.Cell growth as well as Ca2+ uptake was stimulated in a concentration-dependent fashion with FH-LDL compared to control (without LDL).With methylated LDL, which had no capacity to bind LDL receptor, the proliferation and Ca2+ uptake were still significantly increased compared to control.But about 60% of increase over control was reduced by methylation.In analysis of lipid composition, the exposure to FH-LDL elevated free cholesterol contents in SMC membrane.In structural analysis, FH-LDL brought the swelling in the intrabilayer and interbilayer structure compared to control, which well correlated with the increase of free cholesterol in plasma membrane.Heparin or sulfated glycosaminoglycans inhibited the cell proliferation with reduction of free cholesterol in plasma membrane.We concluded that FH-LDL might increase the free cholesterol composition in plasma membrane through LDL receptor-independent pathway, and that change structure of membrane, which affect Ca2+ channel function.
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
Funder: American Heart Association
OpenAlex ID: W1713951051