Quantitative imaging of selenoprotein with multi‐isotope imaging mass spectrometry (MIMS)
Year: 2014
Type: article
Abstract: Multi‐isotope imaging mass spectrometry (MIMS) allows high‐resolution quantitative imaging of protein and nucleic acid synthesis at the level of a single cell using stable isotope labels. We employed MIMS to determine the compartmental localization of selenoproteins tagged with stable isotope selenium compounds in human aortic endothelial cells (HAEC), and to compare the efficiency of labeling (to determine the ideal selenium source) from these compounds: [ 82 Se]‐selenite, [ 77 Se]‐seleno‐methionine, and [ 76 Se]‐methyl‐selenocysteine. We found that all three selenium isotopes appear to be localized in the nucleus as well as in the cytoplasm. For MIMS detection, we compared freeze‐drying to thin layer versus thin sectioning for sample preparation. MIMS provides a unique and novel way to dissect selenoprotein synthesis in cells. Copyright © 2014 John Wiley & Sons, Ltd.
Source: Surface and Interface Analysis
DOI: 10.1002/sia.5625
Authors: S.S. Tang, C. Guillermier, M. Wang, J. C. Poczatek, N. Suzuki, J. Loscalzo, C. Lechene
Institutions: Brigham and Women's Hospital, Harvard University, Chiba University
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Funders: Ellison Medical Foundation, Human Frontier Science Program, National Institutes of Health, Japan Society for the Promotion of Science, National Institute on Aging, National Heart, Lung, and Blood Institute, National Institute of Biomedical Imaging and Bioengineering, National Center for Research Resources
Awards: G2014571086, National Resource for Imaging Mass Spectrometry, NanoSIMS 50L Ion Micro-Analyzer, Neural stem cells in the aging brain, Cell fate and tissue turnover in the aged studied with multi-isotope imaging mass, National Resource for Imaging Mass Spectrometry, Development of novel caged compound bearing off/on switch of light sensitivity, G776136168, The Phathophenotype Landscape of Complex Disease, Cardiomyocyte Lineage and Turnover by Metabolic Fate-Mapping, Arterial Dysfunction: Basic and Clinical Mechanisms, GPx-3 and Peroxide Flux in the Endothelial Cell, G9000087076
OpenAlex ID: W1721887017