Work

TLR9 is dispensable for intestinal ischemia/reperfusion-induced tissue damage.

Year: 2012

Type: article

Abstract: The mortality rate due to intestinal ischemia/reperfusion (IR) remains at 60-80%. As toll-like receptor (TLR) 4 has been shown to be critical for IR injury in several organs, including the intestine, and TLR9 is necessary for IR-induced damage of the liver, we investigated the hypothesis that TLR9 is involved in intestinal IR-induced damage. Wildtype (C57Bl/6) and TLR9(-/-) mice were subjected to intestinal IR or Sham treatment. Several markers of damage and inflammation were assessed, including mucosal injury, eicosanoid production, cytokine secretion and complement deposition. Although IR-induced injury was not altered, PGE(2) production was decreased in TLR9(-/-) mice. Attenuated PGE(2) production was not due to differences in percentage of lipids or COX-2 transcription. The data indicate that TLR9 is not required for IR-induced injury or inflammation of the intestine.

Source: PubMed Central

Authors: Emily Archer Slone, Michael R Pope, Mary Roth, Ruth Welti, Sherry D Fleming

Institution: Kansas State University

Language: English

Topic: Immune Response and Inflammation

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Institute of Allergy and Infectious Diseases, National Center for Research Resources

Awards: Kansas IDeA Network of Biomedical Research Excellence, Natural Antibodies and Tissue Injury, Epithelial Function in Health and Disease, BRITE Veterinary Student Program