Year: 2011
Type: dissertation
Abstract: Methods Experimental designNine vascularized knee joints were transplanted across a strong major histocompatibility complex (MHC) barrier.The control group I consisted of three rats in which the vascular pedicles were ligated.Six animals in group II had patent vessels.Immunosuppression in the form of FK-506 (Fujisawa Pharmaceutical Co., Osaka, Japan) was administered daily for the duration of the study. Animals and anesthesiaInbred female Dark Agouti rats (DA, genetic expression: RT1 a ) weighing 175-200g were used as donors and male Piebald Virol Glaxo rats (PVG, genetic expression: RT1 c ) weighing 200-250g were the recipient animals (Harlan Sprague Dawley, Madison, WI).One donor rat was required for each transplantation procedure (N=9).Donor rats were anesthetized with pentobarbital sodium at a dose of 35 mg/ kg IP.Following graft harvest they were euthanized with an intracardiac injection R1 R2 R3 R4 R5 R6 R7 R8 R9 10 11 12 13
Authors: Larsen, M., Ritt, M.J.P.F.
Language: English
Subfield: Transplantation
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funder: National Institutes of Health
OpenAlex ID: W1745493191