NMR-based analysis and characterization of minor components in Japanese persimmon (Diospyros kaki) cultivars
Year: 2014
Type: dissertation
Abstract: Diospyros kaki, better known as the Japanese persimmon, is recognized as an outstanding source of biologically active compounds related to many health benefits.About 1000 varieties are estimated to be grown in Japan.However, there are only limited efforts to investigate the metabolites in persimmon fruit compared to other fruits.In addition, the investigations on persimmons have generally been focused on certain selected metabolites (carotenoid, polyphenols, etc.) and the metabolic profiling has not been applied to persimmons for an overview of the chemical compounds.Furthermore, identification of persimmon cultivars has been traditionally performed based on morphological and physiological features and recently genetic methods have been used to distinguish cultivars; however, metabolic profiling for discriminating the cultivars of persimmon has not been reported in the literatures at our knowledge.In the present study, metabolic profiling of persimmon juice was performed by nuclear magnetic resonance (NMR) spectroscopy.Since the main components in persimmon juice, such as water and sugars, make minor components undetectable or detected with relatively poor signal-to-noise (S/N) ratio due to the limitation of dynamic range.Broad Band WET method was developed to analyze persimmon juice as a new NMR method for detecting minor components in complex mixtures.Detailed signal assignments of Japanese persimmon juice (Taishu cultivar.)were carried out using various 2D NMR techniques incorporated with Broad Band WET or WET sequences and individual compounds in persimmon juice were quantitatively evaluated.Furthermore, NMR spectra combining with multivariate statistical analysis were employed to discriminate 5 different Japanese persimmon cultivars.A better discrimination and a more informative detail of metabolic differences among cultivars were achieved by Broad Band WET spectra than using conventional 1 H NMR. On the basis of signal assignments, minor components detected by Broad Band WET method were captured as biomarkers responsible for the cultivar discrimination.Broad Band WET spectra combined with multivariate analysis could be a very useful tool for Japanese persimmon cultivar characterization which will help in selection or modification of better or nutritionally important cultivars combined with genomics researches in the future.
DOI: 10.15083/00006857
Author: 晶楽 劉
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W1745700610