Year: 2012
Type: article
Abstract: Ecklonia cava로부터 얻어진 fucoidan을 분해하는 해양세균은 해수로부터 분리하였다. 이 균주의 조효소는 pH8과 $50^{\circ}C$ 에서 fucoidan을 효율적으로 분해하였다. Crude fucoidanase는 1% (w/v) fucoidan 반응액에서 24시간 내에 약 7.1%를 가수분해하였으며 반응산물로서 endo-type 가수분해에 의한 oligosaccharide를 생산하였다. 16S rRNA 유전자 염기서열분석과 생화학적 시험의 결과로부터 SB 1493균주는 잠정적으로 Pseudoalteromonas sp.로 동정하였다. A marine bacterial strain that degraded fucoidan from Ecklonia cava was isolated from seawater. The crude fucoidanase of this strain efficiently degraded fucoidan at pH 8 and $50^{\circ}C$ . The crude enzyme hydrolyzed 7.1% (w/w) fucoidan within 24 hrs from an 1% (w/v) fucoidan solution and produced oligosaccharides by endo-type hydrolysis as the reaction products. The results of 16S rRNA gene sequence analysis and biochemical tests permitted a tentative identification of strain SB 1493 as a Pseudoalteromonas species.
Source: Journal of Life Science
Authors: Yu-Ri Lee, Jong Min Lim, Ki-Young Kim, Sung-Bae Mun, Inseok Kwak, Jae Hak Sohn
Institution: Silla University
Language: English
Subfield: Aquatic Science
Domain: Life Sciences
SDG: Life below water
Open Access status: bronze
OpenAlex ID: W2027947629