Work

Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay

Year: 2009

Type: book-chapter

Abstract: Epidermal stem cells are of major importance for tissue homeostasis, wound repair, tumor initiation, and gene therapy. Here we describe an in vivo regeneration assay to test for the ability of keratinocyte progenitors to maintain an epidermis over the long-term in vivo. Limiting dilution analysis of epidermal repopulating units in this in vivo regeneration assay at sequential time points allows the frequency of short-term (transit amplifying cell) and long-term (stem cell) repopulating cells to be quantified.

Source: Methods in molecular biology

DOI: 10.1007/978-1-60761-380-0_29

Authors: Lauren R. Strachan, Ruby Ghadially

Institutions: San Francisco VA Medical Center, University of California, San Francisco, University of California System

Language: English

Topic: Hair Growth and Disorders

Subfield: Urology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institute of Arthritis and Musculoskeletal and Skin Diseases