Work

Objective method of comparing DNA microarray image analysis systems

Year: 2004

Type: article

Abstract: Many image analysis systems are available for processing the images produced by laser scanning of DNA microarrays. The image processing system takes pixel-level intensity data and converts it to a set of gene-level expression or copy number summaries that will be used in further analyses. Image analysis systems currently in use differ with regard to the specific algorithms they implement, ease of use, and cost. Thus, it would be desirable to have an objective means of comparing systems. Here we describe a systematic method of comparing image processing results produced by different image analysis systems using a series of replicate microarray experiments. We demonstrate the method with a comparison of cDNA microarray data generated by the UCSF Spot and the GenePix image processing systems.

Source: BioTechniques

DOI: 10.2144/04366bi01

Authors: Edward L. Korn, Jens K. Habermann, Madhvi B. Upender, Thomas Ried, Lisa M. McShane

Institutions: National Institutes of Health, National Cancer Institute

Language: English

Topic: Gene expression and cancer classification

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,415

OpenAlex ID: W174936079

FWCI: 0.947

Cites: 16

Cited by: 26

Related to: 10