Cover Picture: One‐Pot Chemoenzymatic Cascade for Labeling of the Epigenetic Marker 5‐Hydroxymethylcytosine (ChemBioChem 13/2015)
Year: 2015
Type: article
Abstract: The cover picture shows fluorescent tagging of the epigenetic modification 5-hydroxymethylcytosine (5-hmC) along a double-stranded DNA molecule. In their work, Y. Ebenstein, M. Fridman, and co-workers show that 5-hmC sites within mammalian genomic DNA that are found at CpG dinucleotides can be directly labeled in a one-pot chemoenzymatic cascade. First, an enzymatic reaction using the uridylyltransferase from Pasteurella multocida (PmGlmU) generates 6-N3-UDPG. This unique and highly difficult to synthesize molecule serves as a substrate for the β-glucosyltransferase enzyme from T4 bacteriophage for selective glycosylation of 5-hmC sites. This enzymatic cascade results in a glucose attached to the 5-hmC modification, and the sugar has an azide moiety for the incorporation of a fluorescent dye by using copper-free click chemistry. More details can be found in the Communication by Y. Ebenstein, M. Fridman et al. on page 1857 in Issue 13, 2015. (DOI: 10.1002/cbic.201500329).
Source: ChemBioChem
Institution: Tel Aviv University
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W1752918480