Work

Large-Scale Production of Polyoma Middle T Antigen by Using Genetically Engineered Tumors

Year: 1985

Type: article

Abstract: A recombinant plasmid containing a metallothionein promoter-polyoma middle T cDNA fusion was constructed and used to transfect NIH 3T3 cells. Transformed cells expressing middle T were injected into nude mice. Within 3 weeks, each mouse produced tumors containing middle T equivalent to that in 250 to 1,000 100-mm dishes of polyomavirus-infected cells. This middle T, partially purified by immunoaffinity chromatography, retained activity as measured by its ability to be phosphorylated in vitro. The combined approach of fusing strong promoters to genes of interest and utilizing nude mice to grow large quantities of cells expressing the gene provides a quick, inexpensive alternative to other expression systems.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.5.7.1795

Authors: D R Kaplan, B Bockus, T M Roberts, J Bolen, M Israel, B S Schaffhausen