Work

Study of Amyloids Using Yeast

Year: 2012

Type: book-chapter

Abstract: We detail some of the genetic, biochemical, and physical methods useful in studying amyloids in yeast, particularly the yeast prions. These methods include cytoduction (cytoplasmic mixing), infection of cells with prion amyloids, use of green fluorescent protein fusions with amyloid-forming proteins for cytology, protein purification and amyloid formation, and electron microscopy of filaments.

Source: Methods in molecular biology

DOI: 10.1007/978-1-61779-551-0_22

Authors: Reed B. Wickner, Dmitry Kryndushkin, Frank Shewmaker, Ryan McGlinchey, Herman K. Edskes

Institutions: National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases, Uniformed Services University of the Health Sciences

Language: English

Topic: Prion Diseases and Protein Misfolding

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases

Awards: Prions Of Yeast, Biological Roles of Yeast Prions, Prions of Yeast and Anti-Prion Systems