Work

Cardiac Stem Cell Niche, MMP9, and Culture and Differentiation of Embryonic Stem Cells

Year: 2013

Type: book-chapter

Abstract: Embryonic stem cells (ESC) are totipotent, self-renewing, and clonogenic, having potential to differentiate into a wide variety of cell types. Due to regenerative capability, it has tremendous potential for treating myocardial infarction (death of myocardial tissue) and type 1 diabetes (death of pancreatic beta cells). Understanding the components regulating ESC differentiation is the key to unlock the regenerative potential of ESC-based therapies. Both the stiffness of extracellular matrix (ECM) and surrounding niche/microenvironment play pivotal roles in ESC differentiation. Matrix metalloproteinase-9 (MMP9) induces fibrosis that causes stiffness of the ECM and impairs differentiation of cardiac stem cells into cardiomyocytes. Here, we describe the method of ESC culture and differentiation, and the expression of MMP9 and its inhibitor, tissue inhibitor of metalloproteinase-4 (TIMP4) in differentiating ESC.

Source: Methods in molecular biology

DOI: 10.1007/978-1-62703-508-8_13

Authors: Paras Kumar Mishra, Nicholas John Kuypers, Shree Ram Singh, Noel Diaz Leiberh, Vishalakshi Chavali, Suresh C. Tyagi

Institutions: University of Nebraska Medical Center, University of Louisville, National Institutes of Health, National Cancer Institute

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute