Work

Methods for the Study of Dopamine Receptors Within Lipid Rafts of Kidney Cells

Year: 2012

Type: book-chapter

Abstract: There is increasing evidence that G protein-coupled receptor (GPCR) signaling is regulated in lipid raft microdomains. GPCRs and GPCR-signaling molecules, including G proteins and protein kinases, have been reported to compartmentalize in these microdomains. Dopamine D(1)-like receptors (D(1)R and D(5)R) belong to a family of GPCRs that are important in the regulation of renal function. These receptors are not only localized and regulated in caveolae that contains caveolin-1 but are also distributed in non--caveolar lipid rafts which do not contain caveolin-1. This chapter describes detergent- and non-detergent-based methods to obtain lipid raft fractions from renal proximal tubule cells.

Source: Methods in molecular biology

DOI: 10.1007/978-1-62703-251-3_2

Authors: Peiying Yu, Van Anthony Villar, Pedro A. Jose

Institutions: Children's National, George Washington University

Language: English

Topic: Caveolin-1 and cellular processes

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of Diabetes and Digestive and Kidney Diseases