Work

Changes in Gibberellic Acid (GA3) Content in Oryza sativa Due to Paclobutrazol Treatment

Year: 2013

Type: article

Abstract: The objective of this study was to determine the level of plant hormone gibberellic acid (GA3) in paddy due to the treatment of Paclobutrazol (PBZ) treatment using high performance liquid chromatography (HPLC) with UV-vis detection at 208 nm. The separation was achieved using reversed column Crestpak C18 (150 mm x 4.6 mm i.d; 5 µm) at 30 ± 1° C using mobile phase of acetonitrile-water (30:70%; v/v), pH 6.80. The treatment of PBZ with different concentration of 100, 200, 400, and 600 mg/L reduced the concentration of GA3 in paddy. The level of GA3 in paddy treated with 100 mg/L of PBZ did not show significant difference from untreated one. However, the level of GA3 in paddy treated with other concentrations (200, 400, and 600 mg/L) of PBZ was significantly different (P < 0.05) from untreated paddy.

Source: Universiti Putra Malaysia Institutional Repository (Universiti Putra Malaysia)

DOI: 10.14499/jfps

Authors: Bambang Surya Adji Syahputra, Uma Rani Sinniah, S. R. Syed Omar, Mohd Razi Ismail

Institution: Universiti Putra Malaysia

Language: English

Topic: Flowering Plant Growth and Cultivation

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: green

OpenAlex ID: W1764183556