Work

Cell Cycle-Dependent Effects on Deoxyribonucleotide and DNA Labeling by Nucleoside Precursors in Mammalian Cells

Year: 1987

Type: article

Abstract: dCTP pools equilibrated to equivalent specific activities in Chinese hamster ovary cells or in nuclei after incubation of cells with radiolabeled nucleosides, indicating that dCTP in nuclei does not constitute a distinct metabolic pool. In the G1 phase, [5-3H]deoxycytidine labeled dCTP to unexpectedly high specific activities. This may explain reports of replication-excluded DNA precursor pools.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.7.1.532

Authors: J M Leeds, C K Mathews

Institution: Oregon State University

Language: English

Topic: Biochemical and Molecular Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institute of General Medical Sciences, National Institute of Environmental Health Sciences

Awards: G1497661912, PREDOCTORAL TRAINING IN MOLECULAR AND CELLULAR BIOLOGY