Work

Regulation of glucose uptake by muscles. 10. Effects of alloxan-diabetes, starvation, hypophysectomy and adrenalectomy, and of fatty acids, ketone bodies and pyruvate, on the glycerol output and concentrations of free fatty acids, long-chain fatty acyl-coenzyme A, glycerol phosphate and citrate-cycle intermediates in rat heart and diaphragm muscles

Year: 1964

Type: article

Abstract: dihydroxyacetone phosphate ratio in the muscles was increased by butyrate and decreased by diabetes and starvation.6.It is concluded: that the oxidation ofpyruvate in rat heart and rat diaphragm muscles may be inhibited in diabetes because of the greater availability of fatty acids and ketone bodies for respiration in this condition; that glycolysis in these muscles is not reversible to any significant extent; that the NADH2/NAD ratio in cell sap is increased by glucose, anoxia, salicylate, fatty acids and ketone bodies, and diminished in allox- an-diabetes and starvation.7. The possibility that the inhibitory effects of diabetes, fatty acids and ketone bodies on pyruvate oxidation are due to inhibition of pyruvate de- hydrogenase by a rise in the acetyl-CoA/CoA ratio is discussed.We thank the British Diabetic Association and the British Insulin Manufacturers for grants that contributed to the cost of these investigations, Evans Medical Ltd. for a generous gift of heparin, and Mrs W. Thorne for skilled technical assistance.

Source: Biochemical Journal

DOI: 10.1042/bj0930678

Authors: PB Garland, PJ Randle

Institutions: University of Cambridge, University of Bristol

Language: English

Topic: Metabolism, Diabetes, and Cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Royal Society